Plasmid

Part:BBa_K4761425

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


pTarget-pykF1

SgRNA(pykF)1 expression plasmid for pykF knockout. Created based on sgRNA(pykF)1 (BBa_K4761025) and pTarget (BBa_K4761107) using overlapping PCR for guide RNA to function in CRISPR-Cas9 double plasmid system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2064
    Illegal NheI site found at 1932
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2064
    Illegal BglII site found at 2094
    Illegal BamHI site found at 1920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
    Illegal NgoMIV site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None